ѧϰ??_????ѧ??>??????ȫ>??????ʶ>?????̲????߲???ҩ?ܻ??á?????

???̲????߲???ҩ?ܻ???

ʱ?䣺2017-09-05 15:33:08???????ݼ?ͼƬ??Դ?ڶ???Ͷ??,??????Ȩ????ϵxuexila888@qq.com ? ??ҪͶ??

?????????????????̲??Ļ??߲????????󡣰??̲?????Ⱥ?????ữ????ôhiv????ҩ?ܻ????????С?????ҷ???һЩhiv????ҩ?ܻ??ã???ҿ?????С??һ?𿴿??ɡ?

????hiv????ҩ?ܻ???

?????????????

???????̲???DZ???ڳ??̲?һ????չ?????ڵ?ʱ??Ҳ????ͬ??

????ָ???????

???????????˴Ӹ?Ⱦ???̲????????ٴ?֢״???һ????չΪAIDS????֢״?ڴ?Լ??8??10?????ң???5%???ҵİ??̲?-1??Ⱦ??û???κ??ٴ?֢״???????ܹ?ά????????????״̬??12?????ϣ?Ŀǰ??Ѵ?17?꣬??֮Ϊ??Ⱦ???̲?-1?ij??ڸ?Ⱦ????չ??;δ???????߽??밬?̲??ں??ƽ????????Ϊ12??18???¡?

????hiv??ⷽ??

???????HIV??Ⱦ????Һ?в?????ԭ?Ϳ???ķ????????????㣬?????ռ?Ӧ?ã????п??????????ͨ????HIv P24??ԭ?Ͳ???????IJⶨ????HIV??Ⱦ????еĵ?λ????Ҫ??Ҳ?????ܵ????ӡ?

??????????

????Ѫ????HIV???????ж?HIV??Ⱦ?ļ??ָ?ꡣ????????Ҫ?????÷?Χ???ɽ?????HIV?????ⷽ????Ϊɸ???????ȷ֤???顣

????ȷ֤?Լ?

????ɸ??ʵ??????Ѫ???ȷ֤??õ???Western blot(WB)?????ڸ÷???Դ????ڽϳ??????????Բ???ҳɱ??߰??????ֻ?ʺ???Ϊȷ֤ʵ?顣???ŵ??????͵??Ĵ?HIV????Լ??????ȵ???ߣ?WB??Խ??Խ???㲻?˶?????Ϊȷ֤ʵ???Ҫ??

????FDA??׼????һ??ɸ??ȷ֤?Լ???-????ӫ??-????(IFA)??IFA??WB?ijɱ??ͣ????Ҳ???Ҳ??Լ򵥣???????????1-1.5Сʱ?ڼ??ɽ??????˷?????Ҫȱ??????Ҫ?????ӫ?????Ǻ?????????רҵ??Ա???۲????н????????ʵ?????޷????ڱ??档????FDA?Ƽ?????WB????ȷ???Ĺ?ѪԱ???????ս??ʱ??IFA?????Ի?????Ϊ׼????????ΪѪҺ?ϸ?ı?׼??

????ɸ??????

????ɸ????????Ҫ???ڶԹ?ѪԱ????ɸ?飬???Ҫ???????㣬?ɱ????????????????????졣2012?꣬????????Ҫ??ɸ?췽????Ȼ??ELISA???????????Ŀ????????Լ??Ϳ???ELISA?Լ???ELISA?кܸߵ??????Ⱥ??????ԣ??????򵥣?????Ҫʵ?????䱸ø???Ǻ?ϴ???????Ӧ?ã??ر??ʺ????????Ҵ??ģɸ??ʹ?á?

????????????ʵ??????һ?ֲ????򵥷??㣬?ɱ??????ļ?ⷽ?????÷????????ͨ???????ж????????Ⱥܸߣ??ر??ʺϷ?չ?й??һ????ɸѡ??ѪԱʱʹ?ã?ȱ???DZ???ʹ????????Ʒ???????Խϲ

????80??????ڷ?չ?????İߵ?ӡ?????(Dot-blot assay)??һ?ֿ???ELISA(Rapid ELISA)?????????ַ?????????Ϊ??㣬???̶??ݣ????????̶?????5-10??????????3?????ڼ??ɽ????????÷???ELISA?Ϳ????????Լ?????öࡣ

????????????ȱ?ݲ????????ǻճĤ????Һ????Լ???(?????)?????ڲ??????߲?????(??????)??𣬻??????߲???????ͨ???ֹ??????????۶?ȡ?????20???Ӽ??ɶ??Եó???????Ŀ???????Լ??????ڼ???ǻճĤ????Һ?????е?HIV-1?ͺ?HIV-2?Ϳ??塣????????Ը??ѯ??⡢??Ը??Ѫ?????뻼?ߵij?ɸ???÷????????ڳ?ɸ??⣬???ɸ??Լ??ⶨΪ?????ߣ?????н?һ??ɸ??ȷ?ϡ?[3]

??????HIV???ԡ?˵?????????ڼ?ⲻ??HIV???壬???Է?????(-)??ʾ??????˵û?и?ȾHIV, Ҫ????ʲôʱ????ģ??ڴ??????ڣ???Ⱦ?ߵ????ڻ?û?в???HIV???壬??û?в?????????HIV???壬??ʱHIV????????Խ????????ڴ?????֮????ģ??????ų???ȾHIV?Ŀ??ܡ?

??????HIV???ԡ?˵?????????ڼ?⵽??HIV???壬???Է?????(+)??ʾ??

??????????????????ء?

??????Ⱦ?????ڴ????ڣ???HIV???????ڵ???????ʱ?仹???????????Ѫ?廹û???γɵ??͵Ŀ??巴Ӧ

???????̲???չ????ĩ?ڣ?????ˮƽ?½?

?????????Dz??????׿???Ľ??淴Ӧ??????????????????ijЩ???Լ?????????????Ѫ????????ֲ??????£???????Բ???һЩ???壬?䷴Ӧ??HIVP24???ĵ??׿???????ķ?Ӧ??????

??????ԭ???

??????ԭ?????Ҫָ?ò??????????????羵??̬?۲졢??????ԭ???ͻ???ⶨ?ȷ??????????걾??ֱ?Ӽ?ⲡ???򲡶?????????ǰ???ַ????Ѷȴ?????Ҫ?????豸??רҵ??????Ա????˽???ԭ????RT-PCR(??ת¼-PCR)???????ٴ???ϡ?HIV-1P24??ԭ????????HIV-1???岻ȷ???򴰿??ڵĸ??????;HIV-1????????ĸ??????Ӥ?????ڵĸ??????????;???Ĵ?HIV-1??ԭ/????ELISA?Լ????????ԣ???HIV-1????ȷ???????ߵĸ?????ϡ?P24??ԭ???һ????ELISA˫??????ķ??Լ????Լ????뾭??SDA??׼ע?ᡢ????Ч???ڣ??????Խ???????????Լ?˵???????к?????ȷ?ϡ?HIV-1P24??ԭ??????????Ϊ30-90%???ý??????ΪHIV??Ⱦ?ĸ?????????ݣ????ܾݴ?ȷ??;HIV-1 P24??ԭ???????ֻ??ʾ?ڱ????????޷?Ӧ???????ų?HIV??Ⱦ???ٴ???һ?㲻??Ϊ?????????Ŀ??

??????????

????HIV???????????HIV??Ⱦ?ĸ?????ϡ????̼?ء?ָ?????Ʒ???????Ч?ж???Ԥ?⼲????չ?ȡ????õ?HIV??????????ⷽ????????ת¼PCRʵ??(RT-PCR)??????????????ʵ??(NASBA)????֧DNA?ӽ?ʵ??(bDNA)?Լ?ʵʱӫ?ⶨ??PCR??????ֵ??ע????ǣ?ÿһ??HIVRNA????ϵͳ????????ͼ???ޣ??????Բ??????Ϳ?????????????λ??RNA???????ʱδ???????????Ʒ?в????в???RNA?????HIV???ᶨ?Լ?????ԣ?ֻ??????????ʵ???????ԣ????????ų?HIV??Ⱦ;HIV?????????ԣ?????Ϊ???HIV??Ⱦ?ĸ???ָ?꣬???ܵ???????HIV??Ⱦ????ϡ?????HIV???ᶨ???????ʱӦ????????????????????ע??ʹ?õ?ʵ?鷽??????Ʒ???????Ʒ???????ⶨ???С????ͼ????ʱ??Ӧע????ͼ????ˮƽ??

????HIV???ᶨ?Լ??Ҳ??????HIV??Ⱦ?ĸ?????ϣ??ڷ???HIV???????ͺͱ???Ȼ????о???Ӧ?á?ͨ??ʹ??PCR??RT-PCR??????ʹ?÷???????ѧʵ????ͨ?õ??????Լ???????????????׻???????ƣ?Ӧ???????????л򳣼??Ķ??꣬Ҳ??ʹ?ø?????????涨?Լ????ʱӦע????Ӧ????????ʹ?õ????????С????⣬???ú????ⷽ???ĸ߶??????ԣ?ʹ?ü??Ϻ?????????⼼???ͷ??????Ը߶Ȼ??ɸ?Ⱦ??Ⱥ?ҿ??????Ե???Ʒ???м??Ϻ????⣬?ɼ?ʱ???ִ????ڸ?Ⱦ?ߡ??÷????ϵ?????Ʒ?ĺ???????и??ߵijɱ?Ч?档

????hiv??Ҫ֢״

????????֢״

??????????֢״??

?????????㷺?ܰͽ??״??ر??Ǿ???Ҹ?͸??ɹ??ܰͽᡣ?ܰͽ??״?ֱ??1???????ң???Ӳ????ʹ?????ƶ???ʱ?䳬???????¡?

????1.????????????ԭ???Ⱥ͵?????

????2.???????????????Խ??͵?????ƣ????

????3.ʳ???½???2?????????ؼ??ᳬ??ԭ???ص?10%??

????4.???????????ֲ???ԭ?????????к????ˮ????ÿ??10?????ϡ?

????5.???١??ɿ????ܡ?

????6.Ƥ??????ǻ????ƽ̹??¡??ķۺ졢?Ϻ?ɫ??ߵ㣬??ʹ??????

????7.?ʡ????????ְװߡ???????????????м?԰ߣ?????Ů???????????????????????״??ࡣ

????8.ͷʹ??????ģ????

??????????????????????֢״???в????ԽӴ?ʷʱ??Ӧ??ʱȥҽԺ??顣

????HIV?????????֢״??û???????ԣ?????ʵ?????У???????ԭ???ܹ?????????֢״????????Ϊ?Լ????????????֢״?Ͷ϶??Լ?Я????HIV??????ֻ?н??п?ѧ??“HIV????/??ԭ???”???ܹ??ó???ȷ?Ľ??ۡ?

??????֢״DZ??

???????̲??????????????һ??????һֱ??֢״??ֱ?ӽ?????֢״?ڡ????̲?DZ???ڵij??̸?????켫????????????ְ??̲??????????͡?ǿ?ȡ?????????Ⱦ;???Լ???Ⱦ???????????߹??ܡ?????״̬??Ӫ??????????䡢?????ҽ???????????????ص??йء?һ??Ϊ6-10?꣬?????д?Լ5-15%??????2-3???ھͽ?չΪ???̲?????Ϊ???ٽ?չ?ߣ????⻹??5%?Ļ????????߹??ܿ???ά????????12?????ϣ???Ϊ???ڲ???չ?ߡ?

???????̲??IJ???֮??

???????ܰͽ?

????Ϊ?⿪??һ???⣬??????ѧ?ҽ????˴??????顣??????֣????ܰͽ?Ϊ???̲??????ṩ??һ?????õı??????ϣ????????̲??????????????????ڵĸò????޷???????????һЩ??Ⱦ???̲?????10???Ѫ????Ϊ?????ߵij??ܰͽ???Ҳ?????Ű??̲???????

??????ѧ?Ҿ?????һ???о????֣????ܰͽ??е?T-CD8?ܰ?ϸ??(ϸ??????T?ܰ?ϸ??)?????ϲ??????֯?е????ֱ???Ϊɱ?ֵ??ܰ?ϸ??ͨ???ܹ????𱻸?Ⱦ??ϸ???????Ʋ??????????ܰͽ??е??????ܰ?ϸ??ȱ????һ???????Ӷ????°??̲??????????в???????????ɢ?????????٣?ʹ??????ء?

????????о?????֤ʵ???³??ܰͽ???T-CD8?ܰ?ϸ??????ȱ?????TGF-βϸ?????ӣ?????????????T-CD8?ܰ?ϸ???Ļ??ԣ?????????˥??

??????????ѧ?ұ?ʾ?????ǵ??о?Ϊ????սʤ???̲??ṩ????˼·??????????TGF-βϸ?????ӣ??޸??????????T-CD8?ܰ?ϸ?????Լ???ǿ??Գ??ܰͽ?????Ƶȡ???Ҳ??????????һ???????????⡣

????????Tϸ??

????????Tϸ????һЩ???̲??????IJ??????á?????ϸ??????ʱ??????Ҳ?ͻ???;ϸ???????????????ͳ?????Ⱦ????Ľ???ϸ????

????????Tϸ????????һ??????????ϸ????????????һЩ???̲??????IJ??????ã???Ҳ??һ???̶???????????Щ?????Ļ??

????CD4ϸ??

???????̲????ḽ????CD4ϸ???ϣ??ٽ???CD4ϸ??????Ⱦ??????һ???˱????̲?????Ⱦʱ?????????ڸ?Ⱦ??????????ϵͳ?????????IJ???ϸ??????????????첡???Ĺ????????̲????᲻?ϸ??ƣ?CD4ϸ?????ƻ???????????ϵͳ?????????µ?????ϸ???????????????ϸ?????????????????????ϸ??????????˱????̲?????Ⱦ????ʹ??Ⱦ???̲????߸о????????ã?û???κ?֢״??????ʱ?????Ѿ????????CD4 ϸ?????ƻ??ˡ?CD4??????Ҫ??????ϸ??????Ⱦ??һ??ʧȥ?˴???CD4ϸ????????????ϵͳ?ͻ??⵽?????Ĵ?????Ը??ּ????ĸ?Ⱦ??ʧȥ?ֿ?????

????Ԥ?????̲??ķ???

???????Ԥ??ĸӤ?????

????Ԥ?????̲?????ĸӤ????????Ч?ķ?????Ԥ?????举Ů??Ⱦ???̲????????и?ȾΣ?յĸ?Ů???羲???????????԰?????Լ??밬?̲???????Ⱦ???й??ԽӴ??ĸ?Ů???ڻ???ǰ??????Ӧ?????????̲??????????⣬?Ѹ?Ⱦ?˰??̲??????ĸ?ŮӦ???⻳?С?һ?????????У?Ӧ????ѯ?о???İ??̲??????ҽ??????????ȫ?̵ĸ?Ԥ??ʩ??ʹ???ִ?????Σ?ս?????͡?

??????Ԥ?????̲??????????

???????????йػ?????ר??????????????Ԥ?????̲??????磬????????δ??????һ????Ч????ȫ?????ܹ㷺????Ԥ?????̲??????????硣?????̲??ǿ???Ԥ???ģ??취??????????߷?????ʶ???????????ɸ?Ⱦ??Σ????Ϊ???????Լ??????˲??ܸ?Ⱦ??

??????η?ֹҽԴ?Դ????

????ҽ????ԱҪ?ϸ?ִ???????????ƶȣ???????ѪҺ???ô??????Ƶ?????Һ??ϴ???ڽ???ע?䡢??????????????ȴ????Բ???ʱ??Ӧ?ϸ???????????????????????????ɲ???֮?䡢ҽ??֮?䴫????????????Լ?????????ҽ?ƹ????У???????????£???????ʹ??Ѫ??Ʒ??ѪҺ??????ʹ??ʱ??Ӧ???????Ҫ????Ѫ??Ʒ??ѪҺ?????ʹ??һ????ע??????????ʹ?ÿڷ?ҩ??


???????Ȥ??

1.???̲???һ?????òŻ???

2.???̲???һ????Ի???

3.???̲??˻????Ի???

4.???˰??̲??ܻ???

5.???̲?һ???????ܻ???

Copyright @ 2006 - 2017 ѧϰ?? All Rights Reserved

ѧϰ?? ??Ȩ???? ??ICP??15032933??-1

ѧϰ?? ѧϰ??

?ص?????
博评网